Comparison of correlation values show minimal acceptable coverage levels for invitrosome experiments.

<p><b>5A</b> Range of correlation values at each coverage level for the pair-wise comparisons of the three replicates for plasmid p4.1 and the three replicates for plasmid p.4.2. The Pearson's correlation values are on the y-axis and the coverage levels are on the x-axis. The range of correlation values for each coverage level is plotted as a whisker plot composed of the 30 pair-wise comparisons at each coverage level. <b>5B</b> Coverage plots for replicate 1 of plasmid p4.1 at coverage levels: 1X, 2.5X, 5X, 10X, 15X and 35X. The colors of the coverage plots correspond to the whisker-plot colors of the same coverage levels in 5A. <b>5C</b> Whisker plot graphs showing the variability of correlation values at low levels of coverage (1X-10X) for all combinations of the kat-group plasmids. In 5A–5C the colors red, orange, yellow, green, indigo and purple represent data from coverage levels of 1X, 2.5X, 5X, 10X, 15X and 35X, respectively.