Overview of project design.

<p>(<b>a</b>), MPCR bias was evaluated using three T cell populations spiked with cells of known TCRs and three controls (with replication) pooled with 33 plasmids inserting known TCR sequences. (<b>b</b>), Comparison of MPCR and 5’RACE. RNA was derived from the PBMC of three healthy donors. It was used to profile the TRB repertoire via both MPCR and 5’RACE. The analyses included a calculation of MPCR/5’RACE reproducibility and comparison of the repertoire characteristics between 5’RACE and MPCR.