Overexpression of NeuroD by injection of lenti-virus in mouse hippocampus.

<p>(A) Fluorescent images of four groups of mice (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0153628#pone.0153628.g005" target="_blank">Fig 5A</a>, sacrificed on day 9) that received different lenti-virus injections (control/ND) and CPP training (saline/morphine injection). Left panel: GFP-BrdU double staining; Right panel: DCX-Tuj-1 double staining (scale bar: 50 μm). (B-C) Measurement of the numbers of EGFP and BrdU labeled neural precursors (N = 8, *p<0.05). (D-E) Measurement of the numbers of DCX and Tuj-1 labeled immature neurons (N = 6, *p<0.05). Data represent mean ± SEM of 6–8 animals in separate experiments. Statistical significance was determined by two-way ANOVA with a Bonferroni test for post hoc comparisons.