HSP27 protein levels in control and patient-derived cells.

<p>(A) Cells were exposed to 50 nM Rotenone for 48 hours. Total protein lysates from 7 control and 7 patient-derived cells were analysed by western blotting to detect endogenous HSP27 protein levels. α –Actin was used as a loading control. (B) Quantitative representation of HSP27 protein levels in untreated and Rotenone treated ONS cells relative to α –Actin levels. (C) Correlation analysis between normalized CyQUANT values (rotenone treated/no treatment) and HSP27 protein levels. The Pearson’s correlation coefficient for control cells is r = 0.84, p<0.05 and patient-derived cells is r = -0.09, p = 0.84) (D) Control and patient-derived cells were nucleofected with no plasmid, GFP-expressing plasmid and HSP27-GFP plasmid and cell number was determined after DMSO and 50 nM rotenone treatment. Controls n = 3 and patients n = 3, data are presented as Mean ± SEM * p<0.05