The relationship between individual levels of HSP27 phosphorylation (Ser-78) in the platelets and the released phosphorylated HSP27 levels induced by collagen in type 2 DM patients.

<p>The baseline levels in unstimulated samples were subtracted from each of the individual HSP27 phosphorylation ratio (phosphorylated-HSP27/total HSP27) and phosphorylated HSP27 levels in the conditioned mixture after platelet aggregation stimulated by 0.3 μg/ml of collagen for 30 min were collected with the platelet counts. Each data about the phosphorylation of HSP27 in platelets and the levels of released phosphorylated-HSP27 were determined by a Western blot analysis using the ImageJ software program and ELISA, respectively. These data were plotted and analyzed by linear regression analysis. (a) Whole subjects (n = 27) were plotted. (b) The residual subjects after excluding what concentration of phosphorylated-HSP27 could not be detected (n = 25) were plotted.