Transgene analyses.

<p>(A) Southern blot analysis of genomic DNA. SacI and BamHI digest of C3(1)Tag mouse genomic DNA (Tag-2724 and Tag 2725) and REAR mouse genomic DNA (REAR-2539 and REAR 2548) probed with the 7.5 kb C3(1)Tag transgene [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155262#pone.0155262.ref016" target="_blank">16</a>] as depicted in (B). Note that the 7.5 kb band representing the entire transgene is present in the C3(1)/Tag mice but absent in the REAR mice. However, an approximately 12 kb band representing the transgene with genomic flanking region is found in both the C3(1)/Tag and REAR mice. (C) FISH analysis of a metaphase spread of splenocytes from the original C3(1)/Tag mouse line using the C3(1)/Tag construct (B) as probe demonstrating transgene on chromosome 6 as previously reported. (D) FISH analyses of chromosome 6 from spleens of normal FVB/N mice, C3(1)/Tag mice, and REAR mice, probed with the same probe as in (C).