TALEN activity in <i>N</i>. <i>benthamiana</i> protoplasts.

<p>A) Schematic of <i>ALS2</i>, indicating the TALEN target site (black triangle). The target site is 306 bp downstream of the stop codon. B) Bar graph depicting the frequency (%) of NHEJ-induced mutations created by the ALS2T1 TALEN with the four different UTRs as well as the no-UTR control. Data for both mRNA and DNA constructs are presented. The asterisk denotes a sample that is significantly different from the no-UTR control (p = 0.0065). Error bars denote standard error. C) Representative NHEJ-induced mutations. In the wild-type (WT) sequence, the TALEN binding site is indicated as underlined, bold text. Representative mutations have the number of deleted bases indicated at the right.