Examples of disruption of genes in human cell lines by ULtiMATE-engineered TALENs.

<p>(<b>A</b>, <b>D</b> and <b>G</b>) Partial sequences of <i>HBEGF</i>, <i>ANTXR1</i>, and <i>LRP1</i> genes in genome containing TALENs binding regions (overlined for TALEN^L and underlined for TALEN^R). Restriction enzyme cutting sites are highlighted in yellow. (<b>B</b>, <b>E</b> and <b>H</b>) Measurement of indel rates in TALENs-treated HeLa and HEK293T cells by restriction enzyme digestion. The uncleaved bands indicate potential indels. Both wild type and cells treated by TALENs targeting <i>ANTXR2</i> gene are used as controls. The percentage of uncleaved band (indicated by red arrow) was measured using ImageJ (<a href="http://rsbweb.nih.gov/ij/" target="_blank">http://rsbweb.nih.gov/ij/</a>). (<b>C</b>, <b>F</b> and <b>I</b>) Sequencing analysis of mutated alleles from 4-6 randomly selected TALENs clones (in HeLa cells). The TALENs binding sites (underlined) and restriction enzyme cutting sites (in yellow) are highlighted. Dashes and red letters indicate deletions and insertions, respectively.