<p>(A) Mutant alleles obtained from the somatic assay; (B) Mutant alleles obtained from G<sub>1</sub> individuals. Sequences of mutant alleles are aligned with the respective wild-type sequences. TALEN target sites in the wild-type sequences are highlighted. The number of individuals carrying identical mutation is indicated to the right of each sequence.</p
<p>A. DNA sequence analysis of Ddx3x and D1Pas1 genes in Neuro 2A cells after transfection with plas...
<p>(<b>A</b>) Nine Left and eleven Right TALEN half-sites were designed to flank the target at a ran...
<p>The total number of clones isolated, analyzed and containing the RE site modified are indicated f...
<p>Green letters designate target sequences of TALENs. Red dashes and blue letters indicate deleted ...
<p>(A) Positions of TALEN targets in the <i>BmBLOS2</i> gene. Open rectangles and numbers above repr...
<p>(<b>A</b>, <b>D</b> and <b>G</b>) Partial sequences of <i>HBEGF</i>, <i>ANTXR1</i>, and <i>LRP1</...
<p>A: Fragment from the <i>TEP1</i> gene showing the target site of the TALEN pair. Nucleotides boun...
<p>A) Schematic of <i>ALS2</i>, indicating the TALEN target site (black triangle). The target site i...
<p>(A) A schematic diagram showing primers for detecting mutations in TALEN target sites of <i>ldlr<...
<p>(A) Schematic representation of the structure of the <i>BmBlos2</i> gene showing part of the 1<su...
Targeted mouse mutants are instrumental for the analysis of gene function in health and disease. We ...
<p>(A) Schematic representation of the <i>BmBlos2</i> gene structure depicting the introns (broken l...
<p>(A) TALEN RNA-injected G0 founders transmit targeted mutations to their offspring. G0 founders we...
<p>A. Exon-intron structure of mouse <i>Pcnxl2</i> (from UCSC genome browser, version m38 mouse geno...
The corresponding wild-type nucleotide sequence (WT) with deduced amino acid sequence is shown in th...
<p>A. DNA sequence analysis of Ddx3x and D1Pas1 genes in Neuro 2A cells after transfection with plas...
<p>(<b>A</b>) Nine Left and eleven Right TALEN half-sites were designed to flank the target at a ran...
<p>The total number of clones isolated, analyzed and containing the RE site modified are indicated f...
<p>Green letters designate target sequences of TALENs. Red dashes and blue letters indicate deleted ...
<p>(A) Positions of TALEN targets in the <i>BmBLOS2</i> gene. Open rectangles and numbers above repr...
<p>(<b>A</b>, <b>D</b> and <b>G</b>) Partial sequences of <i>HBEGF</i>, <i>ANTXR1</i>, and <i>LRP1</...
<p>A: Fragment from the <i>TEP1</i> gene showing the target site of the TALEN pair. Nucleotides boun...
<p>A) Schematic of <i>ALS2</i>, indicating the TALEN target site (black triangle). The target site i...
<p>(A) A schematic diagram showing primers for detecting mutations in TALEN target sites of <i>ldlr<...
<p>(A) Schematic representation of the structure of the <i>BmBlos2</i> gene showing part of the 1<su...
Targeted mouse mutants are instrumental for the analysis of gene function in health and disease. We ...
<p>(A) Schematic representation of the <i>BmBlos2</i> gene structure depicting the introns (broken l...
<p>(A) TALEN RNA-injected G0 founders transmit targeted mutations to their offspring. G0 founders we...
<p>A. Exon-intron structure of mouse <i>Pcnxl2</i> (from UCSC genome browser, version m38 mouse geno...
The corresponding wild-type nucleotide sequence (WT) with deduced amino acid sequence is shown in th...
<p>A. DNA sequence analysis of Ddx3x and D1Pas1 genes in Neuro 2A cells after transfection with plas...
<p>(<b>A</b>) Nine Left and eleven Right TALEN half-sites were designed to flank the target at a ran...
<p>The total number of clones isolated, analyzed and containing the RE site modified are indicated f...