TrxB2 depletion perturbs growth-essential pathways.

<p>(A) Heat-map representation of selected genes with mean expression fold changes >2 in TrxB2-DUC at 6 h post atc treatment (adjusted p<0.02 by one-way ANOVA). (B) Heat-map representation of selected genes with mean expression fold change >3 in TrxB2-DUC at 24 h post atc treatment (adjusted p<0.02 by one-way ANOVA). (C) Impact of extracellular cysteine and methionine on TrxB2 depletion-induced death. Atc-treated TrxB2-DUC cultures were supplemented with 2 mM cysteine, 2 mM methionine or both. CFU were determined at the indicated time points. (D) Impact of partial TrxB2 depletion on susceptibility of <i>Mtb</i> to mitomycin C. TrxB2-TetON-tetO-1C was cultured in 7H9 medium without atc for 3 days to decrease TrxB2 expression before treatment with mitomycin C. Data in (C) and (D) are means ± SD (n = 3 per group) and are representative of three independent experiments.