Pharmacological inhibition of TrxR in primary mesencephalic cultures results in decreased TrxR activity, increased H₂O₂ production and cell death.

<p>(a) Aur (100 and 300 nM) decreases the activity of TrxR after 6 hr of incubation *** = p<0.0001 as determined by 1-way ANOVA (n = 10−18). (b) Subtoxic concentrations of Aur or PQ alone caused minimal increases in H₂O₂ production after 24 hrs. Aur and PQ caused a synergistic increase in H₂O₂ production (n = 4−6). (c) After 48 hrs of treatment there was minimal cell death into the media in either treatment alone. Aur and PQ caused an additive effect on cell death (n = 12−16). Bars represent mean ± SEM. α = p<0.05 compared to 0 nM Aur in same PQ treatment, β = p<0.05 compared to 100 nM Aur in same PQ treatment, χ = p<0.05 compared to 0 µM PQ in same Aur treatment, φ = p<0.01 compared to 100 µM PQ in same Aur treatment as determined by 2-way ANOVA.