Additional files 4: Figure S4. of Increased tauopathy drives microglia-mediated clearance of beta-amyloid

T5x mice exhibit increased astrogliosis. (A, B) Immunohistochemical analysis and IMARIS quantification of GFAP-labeled astrocytes reveal elevated numbers within CA1 of the hippocampus (WT p = 0.13; 5x p = 0.03; Tau p = 0.04) and the parietal association cortex (WT, Tau p < 0.0001; 5x p = 0.0002), but no differences within the dentate gyrus. (C) Western blot analysis of GFAP in cortical fractions corroborate these findings by demonstrating a significant increase in GFAP between T5x and both WT and Thy-Tau22 groups (WT p = 0.006; 5x p = 0.10; Tau p = 0.002). Data are represented as mean ± SEM of optical density (O.D.), n ≥ 8 mice/group. * Indicates p < 0.05 for both ANOVA and Fisher’s protected least-significant difference (PLSD) post hoc tests with significance versus all other groups, whereas *over a bar indicates significance between 2 or 3 particular groups. Scale Bar = 100 μm. (PDF 11243 kb