Additional file 6: of Differential cytotoxicity induced by the Titanium(IV)Salan complex Tc52 in G2-phase independent of DNA damage

Cell cycle distribution profile of cells after 6 h of treatment. Representative flow-cytometry histograms from data presented in Fig. 4. For better visibility, surface of the graph from Ctr sample (black line) is dotted. A: Cell-cycle distribution of HeLa cells after 6 h incubation with Titanium(IV)salan complexes Tc52 and Tc53 30 h after treatment start. Only 10 μM Tc52 shows significant impact on cell-cycle profile, i.e. severe reduction in G1 and increase of the subG1 fraction. B: Cell-cycle distribution of VH7 normal fibroblasts after 6 h incubation with Titanium(IV)salan complexes Tc52 and Tc53 30 h after treatment start. Samples exposed to 5 μM and 10 μM Tc52 display major reduction in number of cells in G1 concomitant with a mild increase in G2-phase. SubG1 fraction is not significantly elevated. (PDF 1086 kb