Additional file 2: of Differential cytotoxicity induced by the Titanium(IV)Salan complex Tc52 in G2-phase independent of DNA damage

Cell viability assay with HeLa tumor cells (squares) and VH7 normal fibroblasts (circles). Cells were exposed to increasing concentrations of Tc52 (filled) or Tc53 (open) titanium(IV)salan compounds solubilized in DMSO and incubated for 48 h. Subsequently, medium was replaced by fresh medium containing 9 μg/ml resazurin and cells were further incubated. Viability is expressed as % of solvent control. Whereas Tc53 is non-toxic in the tested concentration range, Tc52 impairs viability in HeLa and VH7 cells with an EC20 of 3 μM and 1 μM, an EC50 of 6 μM and 3 μM, respectively, and an EC80 of about 10 μM for both. (PDF 1085 kb