Comparison between relative expression measured with RNA-Seq and RT-qPCR.

<p>Relative expression measured with RNA-Seq and RT-qPCR for 6 genes including 3 glutathione S-transferases (Calfi Delta I, Calfi Sigma VI, Calfi mGST3 III) and 3 candidate reference genes (EFA, 16S, actin) in <i>C</i>. <i>finmarchicus</i> adult females feeding on control <i>Rhodomonas</i> sp. or on one of two experimental diets (LD and HD of <i>A</i>. <i>fundyense</i>) for 2 and 5 days (n = 36). In A) relative expression as reads per kilobase per million mapped reads (RPKM) (Log₂) obtained from RNA-Seq are compared with Cq-values from RT-qPCR (n = 36). The line is a least-squares linear fit to the data (R² = 0.879, P<0.0001). In B) relative expression as fold change difference (Log₂) between the experimental and control treatments (n = 24) with genes significantly regulated showing fold-change >1. Calfi-Delta I (in green) and Calfi-Sigma VI (in red) were the only genes showing significant differential expression. Calfi-Delta I was down-regulated exclusively in the HD and Calfi-Sigma VI up in LD and HD treatments. Expression for EFA has been normalized with actin. The line is a least-squares linear fit to the data (R² = 0.896, P<0.0001).