Quantitative RT-PCR results of selected genes that were differentially expressed in the SSH libraries.

<p>Relative quantification of mRNA expression (fold change) of (A) <i>betaine-homocysteine S-methyltransferase 1</i> (<i>bhmt1</i>, JZ575536), (B) <i>fumarate hydratase</i> (<i>fh</i>, JZ575565), (C) <i>argininosuccinate synthetase 1</i> (<i>ass1</i>, JZ575533), (D) <i>carbamoyl-phosphate synthetase III</i> (<i>cpsIII</i>, JZ575539), (E) <i>superoxide dismutase 1</i> (<i>sod1</i>, JZ575606), (F) <i>ceruloplasmin</i> (<i>cp</i>, JZ575541), (G) <i>acyl-CoA desaturase</i> (<i>acd</i>, JZ575387), (H) <i>ferritin light chain</i> (<i>ftl</i>, JZ575418) and (I) <i>glyceraldehyde-3-phosphate dehydrogenase</i> (<i>gapdh</i>, JZ575429), using <i>β-actin</i> as the reference gene, in the liver of <i>Protopterus annectens</i> after 6 months (mon) of aestivation as compared with the freshwater control (A-F), or 1 day (d) of arousal from 6 mon aestivation as compared with fish aestivated for 6 mon (G-I). Results represent mean + S.E.M. (<i>N</i> = 6). *Significantly different from the corresponding control (<i>P</i><0.05).