Validation of RNA-sequencing data by quantitative real-time PCR.

<p>Representative up-regulated and down-regulated target genes from the RNA-Seq data set were validated with real time quantitative PCR using the same samples used for RNA-Seq assays (*<i>p</i><0.05 two-tailed t-test, vs. respective control, n = 4–5). Values represent mean (+/-) SEM of relative gene expression changes vs. 18S housekeeping gene, calculated by the ΔΔCt method. Human alveolar macrophages were stimulated in the presence or absence of LPS for 6 h.