Impact of Vav1^R63W on T cell functions.

<p>(A, upper panels) Representative dot plots of CD4 and CD8 T cells in the lymph nodes (LN) of WT (n = 10) and Vav1^R63W (n = 8) mice. Graphs show absolute numbers of the indicated populations. (A, lower panels) Representative dot plots showing CD44 and CD62L expression on CD4 T cells in the LN of WT and Vav1^R63W mice. The histogram shows the mean percentages of activated CD4⁺CD44^highCD62L^low cells. Data are representative of two independent experiments. (B) CD4⁺CD62L^high T cells from WT (n = 7) and Vav1^R63W (n = 7) mice were stimulated with anti-CD3 and anti-CD28 antibodies for 48h. Histograms show the mean concentration of IL-10, IL-4, IFN-γ and TNFα in the supernatants. (C) Representative analysis of the expression of Foxp3 and CD25 in CD4⁺ T cells in the thymus (upper panels) and the LN (lower panels) of WT (n = 10) and Vav1^R63W (n = 10) mice. Histograms show mean percentages of CD4⁺Foxp3⁺ CD25⁺ and CD4⁺Foxp3⁺CD25^- T cells. The data are from two independent experiments. (D) The suppressor activity of CD4⁺CD62L⁺CD25^bright Treg cells was measured using assays of suppression of T cell proliferation. Treg cells from WT or Vav1^R63W mice were co-cultured with WT effector T cells at various ratios. Results represent the % of inhibition and are representative of six independent experiments. ■: Vav1^R63W mice; □: WT mice; *p≤0.05; **p≤0.01; ***p≤0.001.