Examination of RAGE shedding using immunoprecipitation.

Yunqian Peng (3130083)
Naftali Horwitz (3130086)
Edward G. Lakatta (91639)
Li Lin (111038)

Publication date

September 2016

DOI

10.1371/journal.pone.0153657.g003

Abstract

<p>A549 cells were transfected with FLAG-tagged hRAGE, mRAGEFL, and mRAGEV4 expression vectors. Cell culture medium was collected 16 h post-transfection and immunoprcipitated with anti-FLAG antibodies. The cell lysates (A) and immunoprecipitants (B) were resolved with SDS gel and western blotted with anti-FLAG antibodies conjugated with HRP. * marks the resolved mRAGEFL and mRAGEV4, and β-actin level in the cell lysates was used as the loading control for cell lysates.</p

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Examination of RAGE shedding using immunoprecipitation.

Abstract

Extracted data

Examination of RAGE shedding using immunoprecipitation.

Abstract

Extracted data

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