LPS enhances secretion of AM, IMD, and CGRP by NR8383 cells.

<p>NR8383 cells were incubated in fresh culture medium with or without LPS (1 μg/ml). At indicated time points, the concentration of A) AM, B) IMD, and C) CGRP in the media was measured using colorimetric competitive ELISA. Data is presented as mean±SEM (N = 5). **, p≤0.01, compared to untreated; §§, p≤0.01, compared to untreated at 1 h; ¶¶, p≤0.01, compared to LPS at 1 h (Mann-Whitney test). Raw data are available as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163483#pone.0163483.s001" target="_blank">S1 File</a>.