CGRP decreases LPS-induced IL-1β mRNA levels.

<p>NR8383 cells were treated for 24 h with LPS (100 ng/ml) with or without AM, IMD, or CGRP (each at 100 nM). A), C), E) Messenger RNA expression of pro-IL-1β, IL-6, and IL-10 was measured by RT-qPCR. Data is presented as relative mRNA expression, normalized to LPS-treated cells. Bar represents median. N = 4 (N = 12 for untreated). *, p≤0.05; ***, p≤0.001 (Mann-Whitney test). B), D), F) Levels of IL-1β (N = 5), IL-6 (N = 4), and IL-10 (N = 5) in the culture media were measured by ELISA. Bar represents median. *, p≤0.05; **, p≤0.01 (Mann-Whitney test).