PL-mediated modulation in LPS-induced signaling.

<p>A) BMDMs were pulsed with medium alone or PL, LPS, PL+LPS. Extracts from BMDMs pulsed for mentioned period of time were electrophoretically separated, blotted and probed with Ab specific for phospho NFκB p65 (p65), p38, JNK, and ERK, or acetyl- NFκB p65, while anti- NFκB p65 anti-ERK as controls. Data shown here are representative of three independent experiments. B) Relative effects of PL, LPS, PL+LPS exposure on NFκB activation was measured in J774 macrophage cell line. FAS stimulation was used as a positive control. Data are normalized to luciferase activity in control J774 cells. Data indicates difference of values over their respective baseline control level in unstimulated (US) samples expressed in arbitrary units. C) BMDMs were pulsed with medium alone or LPS (10 ng/ml), SKF (10 µM) +LPS (10 ng/ml). Extracts from BMDMs pulsed for mentioned period of time were electrophoretically separated, blotted and probed with Ab specific for phospho NFκB p65 (p65), and ERK1/2, while anti- NFκB p65 anti-ERK as controls. Data shown here are representative of two independent experiments.