The M78 cytoplasmic C-tail is required for rapid, constitutive endocytosis.

<p>Hela cells were transfected with plasmids expressing HA-tagged M78, either wild type (wt) or C-terminally truncated (CΔ155). 1 day post-transfection, cells were fed primary rabbit anti-HA antibody (1hr, 37degs) then stained with secondary anti-rabbit IgG either before (pre-perm. AF⁵⁹⁴ conjugate) or after (post-perm. AF⁴⁸⁸ conjugate) permeabilisation. Confocal images are shown for AF⁵⁹⁴ (red), AF⁴⁸⁸ (green) or the merged channels.