A rabbit polyclonal antibody detects an AGS3 post-translational modification in HEK293 and HeLa cells.

<p>DNA and siRNA transfections were performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008877#pone-0008877-g001" target="_blank">Figure 1</a>. (A) In HeLa cells, exogenously over-expressed untagged-AGS3 as well as endogenous AGS3 exhibited three differently migrating bands (indicated by asterisks). (B) Characterization of the putative AGS3 bands using lysates collected from HeLa cells transfected with AGS3 siRNAs. The presence of three differently migrating bands which are sensitive to the AGS3 siRNAs is also evident in HEK293 cells (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008877#pone-0008877-g001" target="_blank">Figures 1C, 1E</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008877#pone-0008877-g002" target="_blank">2A</a>). (C) Analysis of the band pattern of an HA-tagged AGS3 expressed in HeLa cells. (D) Comparison of the mobility between recombinant AGS3 expressed in <i>E. coli</i> and endogenous AGS3 from HeLa cell lysate.