Redistribution of the medial/trans Golgi membrane marker 1,4-GT:CFP in cells expressing rabbit calicivirus RdRps.

<p>RK-13 cells were transiently transfected with an expression construct coding for 1,4-GT:CFP (a), co-transfected with constructs coding for 1,4-GT:CFP and RHDV polymerase:myc (b–d) or 1,4-GT:CFP and RCV polymerase:myc (e–g). Cells were fixed 24 h after transfections and recombinant proteins were immunostained using anti-myc antibodies (shown in red) and anti-GFP/CFP antibodies to visualise 1,4-GT:CFP as a Golgi membrane marker (shown in green). DAPI was used to stain cell nuclei (shown in blue). In cells expressing RHDV and RCV RdRps (b and e, respectively), a striking redistribution of the Golgi membrane marker was observed (c–d and f–g, respectively) as compared with the normal Golgi structure in transfected control cells that expressed the 1,4-GT:CFP fusion protein only (a).