miR-181d promotes wild-type HeV infection.

<p>(A) HeV titres of supernatants from HeLa cells infected with HeV (MOI 1) for 24 h, 72 h post-transfection with miRNA agonists. (B) Percentage of the HeLa cells infected with HeV as in (A), and (C) corresponding virus P-antigen levels per infected cell, were quantitated by automated immunofluorescence imaging. (D) Immunofluorescence microscopy images showing HeLa cells treated as in (A) and stained with phosphoprotein-specific (HeV) antibody (green) and DAPI nuclear stain (blue). **p ≤ 0.01, ***p ≤ 0.001.