MEV infection regulates cellular miRNAs including miR-181b.

<p>(<b>A</b>) Expression of selected miRNAs in F81 cells infected with MEV (MOI = 1) and in uninfected controls were tested for validation by qPCR using a specific primer for each miRNA. Fold increase/decrease was calculated based on endogenous control U6 small RNA normalization. (<b>B</b>) qPCR was used to detect the expression levels of miR-181b at 0 h, 6 h, 12 h, 24 h and 36 h after MEV infection, normalized to U6 small RNA. Data are from 3 independent experiments (mean ± SD). Statistical significance was analyzed by Student’s <i>t</i> test; * <i>P</i><0.05; ** <i>P</i><0.01; *** <i>P</i><0.001.