Translational suppression of CVA21 occurs in the presence of high endogenous miRNAs.

<p>(A) Viability 48 hrs. post infection with MOI = 1.0 recombinant CVA21s in the presence of 200 nM control or miR-142 mimic. (B) Viability 48 hrs. post infection with MOI = 1.0 in a panel of hematopoetic or control cell lines or in H1-HeLa cells transfected with 200 nM control or miR-142-3p mimics. (C) Infectious titer in cell supernatant in (B), titrated on H1-HeLa cells and assayed 72 hrs. post infection. (D) Fold decrease in CVA21 RNA 48 hrs. post infection at MOI = 1.0 of hematopoetic cell lines or in trans supplemented miRNA mimic. Fold decrease normalized to GAPDH expression. (E) Relative CVA21 genome copies in singly or doubly infected MEC-1 cells as quantified by qPCR. Primers and probes in 2A region of CVA21 or miRT region of CVA21 genome quantified total or miRT viral genomes, respectively. (F) Relative miR-142-3p abundance in a panel of cell lines. Small RNA was extracted from cells and reverse-transcription and qPCR analysis of miR-142 3p was performed. Abundance correlated to expression in H1-HeLa cells (per 5 ng small RNA). *p = <.01 vs. H1-HeLa + Control; ND- none detected.