hCDC-derived exosomes promote HUVEC migration without affecting proliferation.

<p>(A) The migratory response of HUVECs following hCDC coculture was studied using a 4 hour transwell migration assay. HUVECs cocultured with lentiviral Scrambled control (Scr) CDCs had increased migration as hen compared to endothelial cells cocultured with lentiviral:nSMase2 KD CDCs (57 vs 41%) and media control (n = 3). (B) HUVECs cocultured with Scr CDCs and pulsed with BrdU demonstrated no change in proliferation compared to those cocultured with nSMase2 KD CDCs (% BrdU+ cells/total number of DAPI+ cells; n = 3). *p<0.05 by one-way ANOVA (Tukey’s post hoc test). Data are presented as mean ± SEM.