Cell migration and angiogenic effects of CMD <i>in vitro</i>.

<p>(A)Trans-well cell migration assay using HUVECs showed that the number of migrating cells per microscopic field (200x) in response to ^GF-Sca1CMD was significantly higher compared to ^Sc-Sca1CMD as a control (15±2.38 <i>vs</i> 8±2.35, <i>p</i><0.01). Nuclei were stained with DAPI (blue). (B) Tube formation assay on matrigel using HUVECs treated with conditioned medium (CMD) from ^GFSca-1⁺ cells (^GF-Sca1CMD) or ^ScSca-1⁺ cells (^Sc-Sca1CMD). ^GF-Sca1CMD showed significantly higher number of branch points per microscopic field (100x) as compared to ^Sc-Sca1CMD.