Presence of tumor-associated macrophage (TAM) F4/80 and tumor-associated neutrophil (TAN) Ly6G in xenotransplanted mice are suppressed by PLD inhibitors.

<p>(<b>A,C</b>) Immunofluorescence staining of mouse primary breast tumor sections using either a rat anti-mouse F4/80 AlexaFluor 488 IgG antibody specific for TAMs (<b>A</b>) or a rat anti-mouse Ly6G-R-PE IgG antibody specific for TANs (<b>C</b>). Each set of three panels represents results from Xenotransplanted MDA-MB-231 cells in the presence of vehicle, FIPI or VU0155072-2, respectively. Scale bar = 200 μm. (<b>B,D</b>) ImageJ quantification of the negative effect of PLD inhibitors on the total mean fluorescence of each field of view for either F4/80 (<b>B</b>) or Ly6G (<b>D</b>) in primary metastatic breast tumors. Sample sizes were n = 5 for each set of animals used and 6–8 fields were viewed for each section. Statistical analyses for the increases (*, p<0.05, ANOVA) in xenotransplants and the decreases (#, p<0.05, ANOVA) in PLD inhibitors-treated samples.