Membrane localization of PTH1R wild type and mutants.

<p>Membrane fractions of <i>Xenopus laevis</i> oocytes injected with cRNA of myc-tagged wild type (WT) and mutated PTH1R or H₂O were analyzed by Western immunoblotting. As revealed by myc-tag antibody specific signals (as indicated on the right) were detected in preparations of PTH1R wild type and PTH1R/Gly452Gly whereas these protein bands were absent in samples of PTH1R/Trp339stop and H₂O (negative control). Loading of equal amounts of protein is monitored by unspecific double bands (asterisk) in all samples. Quality and amount of cRNA injected into oocytes for heterologous expression of receptors is documented by RNA gel (lower panel).