Immunocytochemical analysis of <i>Xenopus oocytes</i>.

<p>Immunocytochemical analysis of <i>Xenopus oocytes</i> injected with 50 ng of cRNA encoding the wild-type (wt) or mutant GLUT9 performed with rabbit anti-<i>SLC2A9</i> polyclonal antibody. The signal of protein is green while autofluorescent granules in cytoplasm of oocytes give a blue signal. (a) Non-injected oocytes, (b) oocytes injected with wt cRNA GLUT9 variant 1, (c) oocytes injected with wt cRNA GLUT9 variant 2, (d) GLUT9 variant 2 A17T, (e) GLUT9 variant 1 G25R, (f) GLUT9 variant 1 V169M, (g) GLUT9 variant 2 V140M, (h) GLUT9 variant 2 T246M, (i) GLUT9 variant 2 D252H, (j) GLUT9 variant 1 V282I, (k) GLUT9 variant 2 V253I, (l) GLUT9 variant 1 R294H, (m) GLUT9 variant 2 R265H, (n) GLUT9 variant 1 P350L, (o) GLUT9 variant 2 P321L. Scale bar represents 50 µm.