<i>Xenopus</i> oocytes express neuroplastin that facilitates MCT2 expression at the plasma membrane.

<p><b>A:</b> shows the results of RT-PCR performed on mRNA extracted from <i>Xenopus laevis</i> oocytes, liver and thymus as described in the Experimental section. Primers for basigin (Bas), embigin (Emb) and neuroplastin (Np) were used as indicated. <b>B:</b> shows the expression of MCT2 in oocytes with or without antisense mRNA for basigin or neuroplastin detected by immunofluorescence microscopy. Antisense treatment reduces expression at the plasma membrane (PM) but increases intracellular expression (IC). The scale bar is 50 µm. <b>C:</b> shows parallel data for MCT2 expression in a crude membrane fraction derived from the oocyte and detected by SDS-PAGE and Western blotting. Here, scrambled (Scram) antisense RNA is used as an additional control. <b>D:</b> presents parallel data for the transport of [¹⁴C]-L-lactate into oocytes determined over 5 min. Error bars represent the S.E.M. of the number of oocytes indicated.