Additional file 10: Figure S10. of Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation

Lenti-apoE3 transduced the choroid plexus but not the parenchyma cells. A-H) Representative images showing transduction in the choroidal cells of lenti-apoE3 and lenti-EGFP at 4 weeks after intraventricular delivery. A) Merged image showing choroid plexus (CP; scale bar, 100 μm). B) EGFP (green); C) apoE3 (magenta); D) AQP1 (red); E) merged images of B, C and D (scale bar 50 μm). Arrows show apoE3 in choroidal cells in C and E. F-H) cortex (Cx) showing no transduction (no EGFP) (F, scale bar 200 μm) but the presence of apoE3 (G-H). H, scale bar 50 μm. Lenti-apoE3/lenti-EGFP mixture was delivered intraventicularly (3 μL lenti-human apoE (4.06 × 108 TU/ml) and lenti-EGFP (1012 TU/ml) and after 4 weeks the brains were perfusion fixed followed by immunolabeling. (EPS 41288 kb