Migration of HGG cells isolated from neurospheres is inhibited by PT.

<p>(A) Phase contrast and confocal fluorescence imaging of U87vIII cells that were cultured either as (adherent) monolayers or as neurospheres. In the fluorescent confocal images, nuclei are stained with DAPI (blue). Actin cytoskeleton is stained with phalloidin (red). (B) Immunoblot analysis was performed to detect uPAR, Sox-2, EGFR and actin, as a control for load, in extracts of U87vIII cells, isolated from monolayer cultures (Adh) and neurospheres (NS). (C) Representative image of U87vIII cells, isolated from neurospheres, which migrated to the underside surface of Transwell membranes. (D) U87vIII neurospheres were isolated without disruption and added to upper chambers of Transwells. Cell migration proceeded for 4 h towards 1% FBS in the lower chamber. The medium was supplemented in both chambers with PT (0.5 μg/mL) or vehicle (-PT). Migration is shown relative to vehicle-treated control well (mean ± SEM; n = 3; ***<i>p</i><0.001).