Distribution and migration of HNPCs cultured adjacent to BS slices.

<p><b>A</b>. At 2 weeks migration of h nuc (red) and GFP (green)+ HNPCs was found directed both opposite and towards the BS. Note the large fraction of Tuj1 (white)+ HNPCs located at the sphere periphery. <b>B</b>. At 4 weeks the distribution of h nuc (red)/GFP (green) positive cells was more extensive cf to 2 weeks, with densely packed HNPCs found at the graft/host border. <b>C</b>. A stream of migrating h nuc/GFP/GFAP+ cells toward the host was seen. Only at 4 weeks a visible rim (arrowheads) at the donor-host border of cells was found, including very extensive GFAP+ processes (arrows) <b>D</b>. At the sphere core at both time-points densely packed HNPCs nuclei were found (h nuc, red). <b>E</b>. Tuj1 (white)/h nuc (red)-positive cells exhibited immature (arrow) and migrating cell (arrowhead) profiles in the periphery. <b>F</b>. H nuc (red)/Tuj1 (white)+ migrating cells were found at the periphery (arrowheads). <b>G</b>. H nuc (red)/GFAP (white)- expressing migrating HNPCs (arrowheads) were observed with an increasing toward the host. <b>H</b>. GFP/DCX (red)/hTau (white)+ cells were found migrating in parallel in a chain-like formation perpendicular to the BS slice border. <b>I</b>. Neuronal HNPCs, h nuc (red)/Tuj1 (white)+, found within the host tissue. BS = brain stem slice. Scale bar equals 200 µm in A, B; 100 µm in C, 25 µm in D, 50 µm in E–H, 100 µm in I.