Distinct epigenetic landscape at the TR region in classic KS tissues.

<p>(A) Illustration of epigenetic landscape at the TR region (gi|139472801: 137169–137969). Data were presented as line plot in IGV. The signals of AcH3 and H3K27 histone modifications as well as LANA enrichment are overlaid with the signals of Input (baseline). <b>Red</b>: LANA. <b>Grass Green</b>: AcH3. <b>Dark Blue</b>: H3K27me3. <b>Sky Blue</b>: Input. (B) Results of ChIP-qPCR at TR region in classic KS tissues. Samples prepared for ChIP-Seq were kept small quantity (1/5) for ChIP-qPCR assay before library construction. ChIP–qPCR data were normalized by the percent input method (signals obtained from ChIP were divided by signals obtained from an input sample). Data are presented as mean±SD. (C) Results of ChIP-qPCR at TR region in <i>in vitro</i> cultured cell lines (iSLK.219, BCBL-1 and BC3).