Epigenetic changes and factor accumulation at sites within the <i>COBLL1</i> locus during the 3CHT A13 time-course.

<p>(<b>A</b>) UCSC genome browser overview of <i>COBLL1</i> genomic locus showing ChIP-seq tracks for EBNA3A-TAP, EBNA3C-TAP, the TSS (horizontal arrow) and CpG islands. Primer locations for ChIP-qPCR (<i>COBLL1</i> peak, control and TSS) are indicated (vertical arrows below UCSC genes track). (<b>B-H</b>) ChIP for H3K9ac (<b>B</b>), H3K27ac (<b>C</b>), H3K4me3 (<b>D</b>), H3K27me3 (<b>E</b>), SUZ12 (<b>F</b>), BMI1 (<b>G</b>) and RBPJ (<b>H</b>) on samples from 3CHT A13 time-course at locations within the <i>COBLL1</i> locus, at <i>GAPDH</i> or <i>myoglobin</i> as indicated. Cells were grown in the absence (-HT) or presence of HT (+HT) and numbers indicate the day of harvest. ChIP values represent enrichment relative to input ± standard deviations of triplicate qPCR reactions for ChIP and input of each sample.