Genome-wide LANA binding sites in classic KS tissues.

<p>(A) The general maps of LANA binding sites on KSHV genome are illustrated. Sequence reads for LANA and Input samples were aligned to the KSHV genome (HQ404500+35TR) and visualized in IGV software. Values shown on the <i>y</i> axis represent the relative enrichment of ChIP-Seq signals and has been normalized according to the calculated normalization factors. The epigenetic maps illustrated in the figure contain information from two cases of classic KS tissues. <b>1#</b>: the first case. <b>2#</b>: the second case. The signals of LANA enrichment were overlaid with the signals of Input (baseline) respectively. <b>Red</b>: LANA. <b>Blue</b>: Input. (B) The distribution of peaks in relation to genes. The identified LANA peaks on the host genome were classified into different groups according to the annotation of peaks by PAVIS. (C) Distances from LANA peaks (-3000 bp to +3000 bp) to TSSs in bins of 100 bp. (D) The number of LANA peaks (-3000 bp to +3000 bp) identified from each case of KS tissues and the number of overlapped peaks. (E) LANA peaks at both the gene body and promoter region of MORC2 gene illustrated by IGV on the GRCh37/hg19. The promoter region of MORC2 gene is illustrated in the box. Bar graph on the right is the result of LANA ChIP–qPCR at the promoter region of MORC2. Data were normalized by the percent input method (signals obtained from ChIP were divided by signals obtained from an input sample) and were presented as mean±SD. The corresponding P value (0.0058) was calculated with Student’s t test.