Integrin expression and cell adhesion to NETs.

<p>(A and B) Relative levels of α5β1 and ανβ3 integrins in human K562 cells (A) and its derived clone K562ανβ3 (B) were determined by flow cytometry analysis using primary monoclonal antibodies HA5 (blue histograms) and LM609 (green histograms) recognizing α5β1 and ανβ3 integrins, respectively, and compared to the corresponding negative control (red histograms). Two independent experiments were performed for each cell line and antibody. (C and D) Cell adhesion assays to NETs in K562 (C) and K562ανβ3 (D) cells. Three independent experiments were performed using different NETs stocks obtained from the stimulation of newly differentiated HL-60 cells in the presence of 10% FBS. Results are expressed as percentage of adherent cells compared to the total number of added cells and mean ± SD of three independent experiments is reported for each condition. Statistical significant differences between NETs coated and each experimental condition is indicated by symbols *, ** and ***, meaning p<0.05, p< 0.01 and p<0.001, respectively.