αvβ5 integrin is required for HCT-8/E11 cell interaction with vitronectin.

<p>(A) Isolated HCT-8/E11 cells were incubated without (Ctrl) or with <i>Sb</i>S (<i>Sb</i>S) in the absence (-) or presence of either anti-αv or -α5 integrin mAbs. Cells were then seeded on vitronectin (3,15 µg/ml). Cell adhesion was evaluated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s2" target="_blank">Methods</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s3" target="_blank">Results</a> are expressed as the percentage of cell adhesion compared to untreated cells (Ctrl). Data represent the mean+SD of 3 separate experiments. (B) HCT-8/E11 cells were transfected with anti -αv-integrin siRNAs (SiRNAαv) or scramble oligos (SiRNACtrl) for 48 h. Cells were then plated on vitronectin for 2 h. Cell-ECM adhesion was evaluated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s2" target="_blank">Methods</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045047#s3" target="_blank">Results</a> are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. ***P<0.001.