β4 integrin subunit is functionally involved in cell adhesion and spreading of <i>Col17a1^−/−</i> cells.

<p><i>Col17a1⁻</i>^{<b><i>/</i></b><i>−</i>} keratinocytes were transduced with empty pLKO vector (mock) and two different shRNA to β4 integrin subunit (β4kd#1 and β4kd#2). <b>A</b>, The cells were lysed and equal amounts of total protein were immunoblotted with indicated antibodies. <b>B</b>, Confluent layers of <i>Col17a1⁻</i>^{<b><i>/</i></b><i>−</i>} cells were subjected to trypsin/EDTA detachment assay (as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087263#pone-0087263-g001" target="_blank">Figure 1</a>). The percentage of adherent cells are shown as mean ± SEM (number of independent measurements = 3); <i>*p</i><0.05. <b>C</b>, Cells were grown on LN332 coated chamber slides for 30 minutes, fixed and processed for indirect immunofluorescence with an actin antibody. The graph shows the cell area in µm² (<i>n = </i>35). The data are shown as mean ± SEM. *<i>p</i><0.05. <b>D</b>, <i>Col17a1⁻</i>^{<b><i>/</i></b><i>−</i>} keratinocytes were treated with DMSO (Ctrl) or different phospho-FAK inhibitors (PF 573228 [5 µM] and Inhibitor 14 [1 µM]) for 6 hours and thereafter subjected to trypsin/EDTA detachment assay. The data are shown as mean ± SEM (cells of three individuals have been analyzed; number of independent measurements = 5).