Early gene signatures of immune response to recombinant Sm-p80 + ODN vaccination in mice.

<p><b>(A)</b> Differences in DEGs and immune system related mapped genes (y axis) across time-points (x axis) for mouse splenocytes. <b>(B)</b> Venn diagram depicting the number and percentage of significant differentially expressed genes (<i>P</i> <0.01) at 24 hours (blue), 48 hours (yellow), 7 days (red), or 21 days (green) post-vaccination are shown. Genes that are common to multiple time points are shown by the overlap. <b>(C)</b> Heat map analysis for 5 differentially expressed splenocyte genes (rows) common to all time points examined from 24 hours to 21 days (columns) post-rSm-p80 immunization. <b>(D)</b> Immune system related networks derived from RNA transcripts at each time-point post-rSm-p80 + ODN immunization. Differentially expressed genes identified at each time point were imported into IPA, and the list of genes identified from top regulator effects were selected for molecular network interactions. The overall interaction of each network is described as different biological processes at each time point. Differences in expression values are represented as red for higher fold change or blue lower fold change.