Analysis of T lymphocyte populations in <i>Daxx</i>^<i>t-/-</i> mice.

<p>Cells were isolated from the thymus, spleen, and lymph nodes in mutant <i>Daxx</i>^<i>t-/-</i> and <i>Daxx</i>^<i>+/+</i> control mice, stained for CD3 and B220 (A), or CD4, CD8, CD3, and B220 (B and C) and flow cytometric analysis was performed. Percentages represent the fraction of each indicated subpopulation of the lymphocytes gate. CD4 and CD8 expression was analyzed by gating on the CD3⁺B220^- population (C). Data are representative of at least 6 independent experiments.