Sub-populations of lymphocytes in lymphoid organs of MLF1-IP HET mice.

<p>Cells of hematopoietic origin in lymphoid organs and bone marrow were analyzed by flow cytometry. The percentages of key sub-populations are indicated. The experiments were repeated at least 3 times, and representative histograms provided. <i>A. T- and B -cell sub-populations in the spleen and lymph nodes.</i> CD4 and CD8 T-cell sub-populations in WT and HET spleens, lymph nodes and thymus were analyzed by 2-color flow cytometry. The B-cell population in the spleen and lymph nodes was analyzed according to B220 and CD19 expression by 2-color flow cytometry. Percentages are indicated. <i>B. Thymocyte sub-populations.</i> Thymocytes from WT and HET mice were stained with CD4 or CD8 for 2-color flow cytometry, or with lineage markers (CD3ε, CD8β, TCRβ, CD11b, CD45R, B220, Ly6C, Ly6G, Ter-119), CD25 and CD44 for 3-color flow cytometry. Lineage^- cells were gated and analyzed for their CD25 and CD44 expression. <i>C. Lineage-negative bone marrow cell sub-populations.</i> WT and HET bone marrow cells were stained with lineage markers, c-Kit and Sca-1for 3-color flow cytometry. Lineage- cells were gated, and analyzed for their c-Kit⁺Sca-1⁺ stem cell population. <i>D. Pre-B, pro-B, pre-pro-B cell sub-populations and myeloid cell in bone marrow.</i> WT and HET bone marrow cells were stained with B220, CD43, CD19 and AA4.1 and analyzed by 4-color flow cytometry for B220⁺CD43⁺CD19^-AA4.1⁺ pre-pro-B cell (region II in column 2) B220⁺CD43⁺CD19⁺AA4.1⁺ pro-B cell (region I in column 2) and B220⁺CD43^-CD19⁺AA4.1⁺ pre-B cell (region III in column 3) populations. Bone marrow cells were also stained with Mac-1 and Gr-1 (column 4) for analysis of mature Mac-1⁺Gr-1⁺ neutrophils, or stained with Ter-119 and CD71 for analysis of CD71⁺Ter119⁺ erythroid precursor cells.