Overall expression levels of the mouse Sigmar1 splice variant mRNAs.

<p>Expression levels (-1/ΔC(t)) of the mouse Sigmar1 splice variant mRNAs is plotted across tissues and brain regions using MS excel. ΔC(t) values were determined as: Δ<i>C</i>(<i>t</i>)<i>variant</i> − <i>Normalized factor (NF)</i> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174694#pone.0174694.ref033" target="_blank">33</a>] from at least three independent samples in one experiment. PFC: prefrontal cortex. SYBR green qPCR was performed as described in Materials and Methods. All the primer sequences and qPCR conditions are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174694#pone.0174694.s004" target="_blank">S1 Table</a>.