<i>Csmd1</i> RNA and protein expression in <i>Csmd1</i> knock-out and wild-type mice.

<p>(A) Schematic representation of the KO-strategy. A 1 kb genomic region (white lines) of exon1/intron1 was replaced with a selection cassette (grey box). (B) Expression of <i>Csmd1</i> mRNA measured by QPCR in an adult mouse tissue panel. <i>Csmd1</i> is predominantly expressed in brain tissues as compared to peripheral tissues. The highest expression level was identified in areas of the cortex. (C) Depletion of <i>Csmd1</i> mRNA in the cortex was documented by two exon-exon specific QPCR assays. Transcription of exon 1–2 was depleted, while about 20% residual expression could be observed when amplifying exon 32–33. KO mice lacked a protein band of expected size (389 KDa, arrow), as demonstrated by immunoblotting. Signals of lower molecular weight are indicated (a and b). (D) Mapping of RNA-seq reads to the <i>Csmd1</i> locus. RNA sequencing of cortex is shown for 4wild-type (green) and 4 <i>Csmd1</i> KO (red) mice (transcript scale: 0–150 reads). Coverage signals of modified nucleosomes (H3K4me3, H3K4me1 and H3K27Ac) and polymerase-2 binding profiles are shown for the mouse cortex. The 1 kb deleted sequence of <i>Csmd1</i> is highlighted in yellow (upper panel) and blue (lower panel). No RNA reads were mapped to the deleted genomic region in the KO mice. Abbreviations: Cx, cortex; VCx, visual cortex; FCx, frontal cortex; Hipp, hippocampus; Hyp, hypothalamus; Ob, olfactory bulb; Cer, cerebellum; Visc. Fat, visceral fat.