Additional file 1: Figure S1. of Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone

Schematic illustration of the strategy used to construct transfer vector pUC18/EGFP-TKAB-BAC11. The number in circle indicated different cloning steps. ❶: Cloning of EGFP into pUC18 for constructing pUC18/EGFP. ❷❸: TKA and TKB were amplified from DEV CHv and subsequently cloned into pUC18-EGFP to generate pUC18/EGFP-TKAB. ❹: Linearized pUC18/EGFP-TKAB and BAC Mini-F sequence donor pBeloBAC11 were obtained by Sph I digestion. ❺: Transfer vector pUC18/EGFP-TKAB-BAC11 harboring the homologous regions of TK insertion site, mini-F sequence of BAC and a cellular screening marker EGFP was generated after ligation. (PDF 534 kb