Additional file 2: Figure S2. of Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

WB analyzed the expression of UL24-fusion protein and UL54-fusion protein in HEK293T cells. HEK293T cells were transfected with eukaryotic plasmid pCMV-myc, pCMV-myc-UL24, and pCMV-Flag-UL54 respectively. At 48 h post-infection, the 293 T cell extracts were carried out Western blotting analysis, which indicated that myc-UL24 and Flag-UL54 was expressed in 293 T cells and the molecular mass of fusion protein is about 45 KD, 50.5 KD respectively. Primary Abs against myc-UL24 and Flag-UL54 were serums of rabbit against UL24 and mouse against Flag respectively. (PDF 44 kb